Fig. s4

jam3b:Jam3b-mCherry expression during post-embryonic development. (A) Basal epidermal cells of the skin. (B) Superimposed brightfield and fluorescence of image of Jam3b-mCherry expression in lens of a juvenile fish. (C) Melanophores of the stripe. In brightfield (captured by photomultiplier tube; PMT) melanin is evident, contracted by epinephrine to centers of melanophores. Reflecting platelets of sparsely arranged stripe iridophores are also evident (e.g., arrow). Melanophores express mitfa:EGFP, with jam3b:Jam3b-mCherry accumulations at cell peripheries. In contrast to melanophores beneath them, stripe iridophores did not have detectable accumulation of Jam3b-mCherry. Asterisk, lipid droplet in underlying adipocyte. (D) In the interstripe, reflecting platelets of densely packed iridophores are apparent. In brightfield-PMT, accumulations of carotenoid pigments within xanthophores appear black (dashed circles). Iridophores and reflecting platelets are labeled with pnp4a:EGFP and Jam3b-mCherry is evident where cells contact one another. Scale bars, 20 μM (A,C,D), 500 μM (B).