FIGURE

Fig. 6

ID

ZDB-FIG-211105-19

Publication

Eom et al., 2021 - Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Expression and function of jam3b relative to igsf11. (A) Melanophores isolated from larvae (7.5 SSL) and plated onto culture dishes expressed jam3b:Jam3b-mCherry and stained with polyclonal antiserum to Igsf11. (B) Fish heterozygous for both igsf11 and jam3b (here igsf^11utr15e1 and jam3b^vp8re2) had stripe breaks (upper) rather than wild-type stripes, as observed in fish heterozygous for both gja5b and jam3b^vp8re2 (lower). (C) Jam3b-mCherry and Igsf11-EGFP supported homophilic but not heterophilic adhesion of S2 cells after 60 min in rotary culture. (D) Somatic loss of jam3b activity eliminated most residual hypodermal melanophores that develop in igsf11 mutants (here igsf11^vp15rc5). Insets show higher magnification views including some persisting melanophores at the ventral margin of the flank (lower right). Scale bar, 10 μM (A).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gja5b^t1/+; jam3b^vp8re2/+ igsf11^wp15e1/+; jam3b^vp8re2/+
Observed In:	melanophore stripe
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 476, Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M., Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes, 314-327, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.