Figure 6

Three out of five tested anti-epileptic drugs (AEDs) rescue epileptiform brain discharges but not seizure-like behavior. (A) Quantification of seizure-like behavior at 3 dpi after incubation of VHC- and KA-injected larvae with commonly used AEDs or DMSO control for 24 h. None of the tested compounds decreased seizure-like behavior (tail twitching). Values were normalized to KA-injected controls treated with 1% DMSO as 100%. Total number of larvae per condition: VHC + DMSO n = 20, KA + DMSO n = 18, KA + CBZ n = 15, KA + TGB n = 15, KA + TOP n = 15, KA + VPA n = 15, KA + LEV n = 12. Data are presented as mean ± s.d. Statistical analysis was performed using ordinary one-way Anova with Bonferroni's multiple comparison test (^****p < 0.0001). (B) Mean power spectral density (PSD) values from LFP recordings at 3 dpi plotted per condition over the 1–60 Hz region for VHC- and KA-injected larvae after incubation with AEDs or DMSO control for 24 h. Values were normalized to KA-injected controls treated with 1% DMSO as 100%. CBZ (^*p = 0.04), TGB (ns, p = 0.05) and TOP (^*p = 0.03) decreased epileptiform brain activity in KA-injected larvae (VHC ^**p = 0.001), while VPA and LEV did not have any effect. Total number of larvae per condition: VHC + DMSO n = 18, KA + DMSO n = 38, KA + CBZ n = 15, KA + TGB n = 14, KA + TOP n = 14, KA + VPA n = 11, KA + LEV n = 12. Data are presented as mean ± s.d. Statistical analysis was performed using ordinary one-way Anova with Bonferroni's multiple comparison test. CBZ, carbamazepine; TGB, tiagabine; TOP, topiramate; VPA, valproic acid; LEV, levetiracetam.