Figure 6
- ID
- ZDB-FIG-210821-20
- Publication
- Liu et al., 2021 - Insulin-producing β-cells regenerate ectopically from a mesodermal origin under the perturbation of hemato-endothelial specification
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(A) Constructs of etsrp:Cre and ?3.5ubb:LOXP-EGFP-LOXP-mCherry (ubi:Switch). (B, C) Quantification of the pancreatic or ectopic ?-cells with or without etsrp-positive mesodermal origin in control or etsrp morpholino (MO)-injected larvae at 3 dpf. *p=0.0169, ***p=0.0002, and ****p<0.0001 (?idák?s multiple comparisons test); n=33 (control) and 30 (etsrp MO). Data are represented as the mean ± SEM. (D?E???) Representative confocal images of ectopic ?-cells and etsrp-positive lineage-traced cells in control or etsrp MO-injected Tg(ins:Flag-NTR);Tg(ubi:Switch);Tg(etsrp:Cre) zebrafish larvae at 3 dpf after ?-cell ablation by MTZ at 1?2 dpf, displaying ?-cells in cyan with immunostaining for insulin and lineage-traced cells derived from etsrp-expressing mesodermal cells in red from the Cre-recombined ubi:Switch. The selected area in a dashed square in (E) is magnified in split (E?, E??) and merged (E???) channels, respectively. Arrowheads point to ectopic ?-cells derived from the mesoderm (E?E???). (F) Constructs of etsrp:Cre and ins:LOXP-mCherry-LOXP-Hsa.HIST1H2BJ-GFP (ins:CSH). (G?G???) Representative confocal images of ectopic ?-cells derived from the etsrp-expressing lineage in etsrp MO-injected Tg(ins:Flag-NTR);Tg(ins:CSH);Tg(etsrp:Cre) zebrafish larvae at 3 dpf after ?-cell ablation by MTZ at 1?2 dpf, displaying ?-cells in magenta with immunostaining for insulin and lineage-traced cells derived from etsrp-expressing mesodermal cells in nuclear green from the Cre-recombined ins:CSH. The selected area in a dashed square in (G) is magnified in split (G? and G??) and merged (G???) channels, respectively. Scale bars = 20 ?m (D, E, and G) or 10 ?m (E??E??? and G??G???). Anatomical axes: D (dorsal), V (ventral), A (anterior), and P (posterior).
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