FIGURE

Figure 6

ID

ZDB-FIG-210810-61

Publication

Madakashira et al., 2021 - Nuclear Organization during Hepatogenesis in Zebrafish Requires Uhrf1

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Figure 6

uhrf1 loss disrupts hepatocyte nucleoli and laminB2 organization. (A). Control and uhrf1^−/− hepatocyte nuclei scored at 80, 96, and 120 hpf for the number of nucleoli per hepatocyte. (B). Differential expression of nucleoli-associated genes in 120 hpf uhrf1^−/− livers, nc: not significantly changed (C). Control and uhrf1^−/− hepatocyte nuclei scored at 80, 96, and 120 hpf for the presence of normal lamina. (D,E). Confocal images comparing the immunostaining of LaminB2 in 120 hpf uhrf1^−/− and dnmt1^−/− livers with their respective WT controls. (F). Quantitative changes in LaminB2 staining in 120 hpf uhrf1^−/− and dnmt1^−/− hepatocyte nuclei. The numbers of LaminB2 positive nuclei are indicated below each bar. White arrows mark LaminB2 positive hepatocyte nuclei. All analyses were performed on 2 or 3 clutches, with minimum 3 livers analyzed per clutch. ** p < 0.005, *** p < 0.0005, **** p < 0.00005. Magnification: 63×, Scale bar: 7 μm.

Expression Data

Genes:	dennd5b exoc4 hmgb3b phf6 rpf1 rpl3 rpl23a
Antibody:	Ab2-lmnb2
Fish:	mss4Tg mss8Tg dnmt1^s904/s904; mss4Tg uhrf1^{hi272Tg/hi272Tg}; mss4Tg uhrf1^{hi272Tg/hi272Tg}; mss8Tg
Anatomical Terms:	hepatocyte nuclear lamina liver
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^s904/s904; mss4Tg uhrf1^{hi272Tg/hi272Tg}; mss4Tg uhrf1^{hi272Tg/hi272Tg}; mss8Tg
Observed In:	hepatocyte nuclear lamina hepatocyte nucleolus hepatocyte nucleus liver
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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