FIGURE

FIGURE 1

ID

ZDB-FIG-210718-23

Publication

Łysyganicz et al., 2021 - Loss of Deacetylation Enzymes Hdac6 and Sirt2 Promotes Acetylation of Cytoplasmic Tubulin, but Suppresses Axonemal Acetylation in Zebrafish Cilia

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FIGURE 1

1.5-year-old zebrafish do not display any obvious external phenotype. (A) Pictures of single mutant fish were taken at 1.5 year – single mutation did not cause any visible abnormalities. (B) Comparison of wild type and mutated sequences for hdac6 and hdac10 and sirt2 genes, the first changed amino acid caused by frameshift is indicated in red. (C) Schematic representation of proteins of interest – the red mark shows the premature stop codon caused by the mutation. (D) External phenotypes of double mutants and triple mutants. (E) qPCR results showing expression levels for hdac6, hdac10, and sirt2, relative to b-actin level and normalized to wild type (t-test ***p < 0.005).

Expression Data

Genes:	hdac6 hdac10 sirt2
Fish:	WT hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdac6^sh400/sh400 hdac10^sh401/sh401 sirt2^sh626/sh626 hdac6^sh400/sh400; sirt2^sh626/sh626 hdac10^sh401/sh401; hdac6^sh400/sh400 hdac10^sh401/sh401; hdac6^sh400/sh400; sirt2^sh626/sh626
Observed In:	whole organism
Stage Range:	Day 5 to Adult

Phenotype Detail

Acknowledgments

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