FIGURE

Fig. 8

ID

ZDB-FIG-210712-26

Publication

Buono et al., 2021 - Analysis of gene network bifurcation during optic cup morphogenesis in zebrafish

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Fig. 8

Optic cup architecture and size quantification in candidate gene crispant embryos.

a–o Representative optical sections through the optic cup of crispant 24 hpf embryos. Phalloidin/DAPI staining reveals tissue organization of control embryos injected only with Cas9 (a), with sgRNAs directed against rx3 (b), or with sgRNAs for the different candidate genes (indicated in (c)–(o)). Note that, in contrast to rx3 crispant embryos, the optic cup is formed in all cases. However, quantitative analysis of eye area (p) reveals a significant reduction of optic cup size for all candidates tested with the exception of nr2f1. Individual values (n = 10) are plotted in front of standard box-and-whiskers (Two-tailed T-test); ***=p < 0.001, ****=p < 0.0001. p values: cas9 = 0.161; rx3 = 1.15e−6; crh1a = 8.67e−5; dsp = 1.82e−4; mcm2 = 3.09e−5; mif = 1.05e-9; neurod4 = 9.00e−8; nop58 = 8.09e−6; nr2f1 = 0.053; smad6b = 2.25e−4; tcf12 = 2.71e−9; tead1 = 1.62e−6; tead1 + 3 = 1.15e−6; vgll2 = 9.15e−7; wdr12 = 5.00e−4. Abnormal morphology of the RPE cells (m,n) is indicated (red arrowheads). ov optic vesicle, lv lens vesicle. Bar = 50 µm. Source data are provided as a Source Data file. See also Supplementary Figs. 9 and 10.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + CRISPR1-mcm2 + CRISPR2-mcm2 AB/TU + CRISPR1-mif + CRISPR2-mif AB/TU + CRISPR1-nop58 + CRISPR2-nop58 AB/TU + CRISPR1-utp4 + CRISPR2-utp4 AB/TU + CRISPR1-wdr12 + CRISPR2-wdr12 AB/TU + CRISPR2-tcf12 + CRISPR3-tcf12 AB/TU + CRISPR3-smad6b + CRISPR4-smad6b AB/TU + CRISPR10-neurod4 + CRISPR9-neurod4 AB/TU + CRISPR1-dspa + CRISPR1-dspb + CRISPR2-dspa + CRISPR2-dspb AB/TU + CRISPR1-nr2f1b + CRISPR2-nr2f1a + CRISPR2-nr2f1b + CRISPR3-nr2f1a AB/TU + CRISPR1-tead1b + CRISPR2-tead1b + CRISPR6-tead1a + CRISPR7-tead1a AB/TU + CRISPR1-vgll2a + CRISPR1-vgll2b + CRISPR2-vgll2a + CRISPR2-vgll2b AB/TU + CRISPR1-tead1b + CRISPR1-tead3b + CRISPR2-tead1b + CRISPR2-tead3b + CRISPR4-tead3a + CRISPR5-tead3a + CRISPR6-tead1a + CRISPR7-tead1a
Knockdown Reagents:	CRISPR1-dspa CRISPR1-dspb CRISPR1-mcm2 CRISPR1-mif CRISPR1-nop58 CRISPR1-nr2f1b CRISPR1-tead1b CRISPR1-tead3b CRISPR1-utp4 CRISPR1-vgll2a CRISPR1-vgll2b CRISPR1-wdr12 CRISPR2-dspa CRISPR2-dspb CRISPR2-mcm2 CRISPR2-mif CRISPR2-nop58 CRISPR2-nr2f1a CRISPR2-nr2f1b CRISPR2-tcf12 CRISPR2-tead1b CRISPR2-tead3b CRISPR2-utp4 CRISPR2-vgll2a CRISPR2-vgll2b CRISPR2-wdr12 CRISPR3-nr2f1a CRISPR3-smad6b CRISPR3-tcf12 CRISPR4-smad6b CRISPR4-tead3a CRISPR5-tead3a CRISPR6-tead1a CRISPR7-tead1a CRISPR9-neurod4 CRISPR10-neurod4
Observed In:	optic cup retinal pigmented epithelium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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