FIGURE

Figure 1

ID

ZDB-FIG-210606-160

Publication

Rohrer et al., 2021 - Conditional Loss of the Exocyst Component Exoc5 in Retinal Pigment Epithelium (RPE) Results in RPE Dysfunction, Photoreceptor Cell Degeneration, and Decreased Visual Function

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Figure 1

Histological and TEM analysis of RPE and retinas of wild-type and exoc5 mutant zebrafish larvae. (A) In systemic exoc5 homozygous mutants (exoc5−/−), the photoreceptor outer segments (OS) were shorter compared to wild-type (WT) siblings. (B) Ultrastructural analysis of WT and exoc5−/− mutant RPE using transmission electron microscopy indicate reduced levels of melanosomes in the mutant fish RPE. (C) WT photoreceptors showed tightly stacked disk membranes, while in exoc5−/− mutants, only remnants of outer segments (OS) could be observed. Please note the apparent formation of ectosomes at the base of the mutant photoreceptor OS. Scale bars = 2 μm (B), 800 nm (C). OS, outer segments; ONL, outer nuclear layer; RPE, retinal pigmented epithelium; INL, inner nuclear layer; GCL, ganglion cell layer.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	exoc5^{sa23168/sa23168} (AB/TU)
Observed In:	eye retinal photoreceptor layer retinal photoreceptor layer photoreceptor outer segment retinal pigment epithelium development retinal pigmented epithelium retinal pigmented epithelium melanosome
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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