FIGURE

Fig. 4

ID

ZDB-FIG-210307-61

Publication

Hans et al., 2021 - Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

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Fig. 4

<italic>sox10</italic>-specific Cre activity results in spatiotemporally controlled GFP expression and pigmentation defects along the body.

a Timeline. Cre activity in sox10:Cre-positive animals at 10 hpf elicits recombination (blue box) in developing neural crest cells. At 16 hpf, a heat treatment triggers expression of Cas9-GFP and the subsequent permanent mutagenesis of the tyr target site (green box). Analysis of GFP expression and pigmentation was conducted at 22 and 50 hpf, respectively. b Expression of GFP is detected in neural crest cells at 22 hpf. c, d In comparison to controls (ctrl), GFP-positive embryos (GFP+) display a loss of pigmentation in the anterior head region. Examples shown are representatives across eight experiments showing the same result. A total of >160 GFP-positive individuals and their respective non-GFP-positive siblings were analyzed. Scale bars: 150 µm in b and d; 500 µm in c.

Expression Data

Gene:	GFP
Fish:	ba73Tg; tud118Tg
Condition:	heat shock
Anatomical Term:	neural crest cell
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ba73Tg; tud118Tg
Condition:	heat shock
Observed In:	head pigmentation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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