ZFIN Image: Hans et al., 2021, Fig. 4

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Figure Caption

Fig. 4 <italic>sox10</italic>-specific Cre activity results in spatiotemporally controlled GFP expression and pigmentation defects along the body.

a Timeline. Cre activity in sox10:Cre-positive animals at 10 hpf elicits recombination (blue box) in developing neural crest cells. At 16 hpf, a heat treatment triggers expression of Cas9-GFP and the subsequent permanent mutagenesis of the tyr target site (green box). Analysis of GFP expression and pigmentation was conducted at 22 and 50 hpf, respectively. b Expression of GFP is detected in neural crest cells at 22 hpf. c, d In comparison to controls (ctrl), GFP-positive embryos (GFP+) display a loss of pigmentation in the anterior head region. Examples shown are representatives across eight experiments showing the same result. A total of >160 GFP-positive individuals and their respective non-GFP-positive siblings were analyzed. Scale bars: 150 µm in b and d; 500 µm in c.

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Acknowledgments

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