Fig. 7. Cdx1b regulates expression of ndr2 and lft1 in the dorsal diencephalon. A Images of a 22–24 S embryo that was injected with cdx1b-vivo-MO1 and dextran-fluorescein in its ventral, lateral and dorsal positions. B RNA in situ hybridization was conducted on cdx1b-vivo-4mm MO1-(cdx1b-vivo-4mm) or cdx1b-vivo-MO1-injected embryos to evaluate expression pattern in the dorsal diencephalon using ndr2 RNA probes at 21–22 s stage. C RNA in situ hybridization was conducted on cdx1b-vivo-4mm MO1- or cdx1b-vivo-MO1-injected embryos to evaluate expression pattern in the dorsal diencephalon using lft1 RNA probes at 23–24 s stage. Quantitative data show the percentages of embryos displaying different expression patterns of ndr2 or lft1 in the dorsal diencephalon in cdx1b-vivo-4mm MO1- and cdx1b-vivo-MO1-injected embryos. Embryos (B, C) are shown in ventral view. Scale bar represents 100 μm. Chi-squared test of independence was conducted to compare proportions of embryos with absent ndr2 or lft1 expression patterns in the dorsal diencephalon between cdx1b-vivo-4mm MO1- and cdx1b-vivo-MO1-injected embryos. ∗∗, p < 0.01; ns, not significant. dd, diencephalon; fv, forebrain ventricle; hv, hindbrain ventricle; L, left; mv, midbrain ventricle; R, right.
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