FIGURE

Fig. 5

ID

ZDB-FIG-210131-5

Publication

Facchinello et al., 2021 - Efficient clofilium tosylate-mediated rescue of POLG-related disease phenotypes in zebrafish

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Fig. 5

Altered signalling and mitochondrial dysfunction in <italic>polg</italic> mutants.

A The CREB signalling-targeted gene fosab is upregulated in 4 dpf polg^{sa9574/sa9574} mutants, compared to controls. B The Hif-Hypoxia signalling-targeted gene hbbe3 is upregulated in 4 dpf polg^sa9574/sa95 mutants, compared to controls. C–E Hif-Hypoxia signalling increases also in 6-dpf polg^ia302/ia302 mutants, as shown by the Hif-Hypoxia-responsive transgene HRE:mCherry. E Chart reporting the HRE fluorescence relative intensity (R.I.). F Diagram depicting the oxygen consumption rate (OCR) profile throughout Seahorse assay in 4 dpf wt and polg^{sa9574/sa9574} embryos under basal conditions, FCCP (p-triFluoromethoxyCarbonylCyanide Phenylhydrazone)-induced maximal respiratory capacity stimulation and ROT/AA (Rotenone/Antimycin A)-mediated inhibition. Three independent experiments were performed (N = 20). G Quantification of basal respiration in wt and polg^{sa9574/sa9574} mutants (N = 20, in triplicate).

Expression Data

Genes:	fosab hbbe3
Fish:	WT polg^{sa9574/sa9574}
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	polg^{sa9574/sa9574}
Observed In:	aerobic respiration hypoxia-inducible factor-1alpha signaling pathway positive regulation of CREB transcription factor activity whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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