Figure 2

BRS-28 reduces the number of neutrophils and macrophages migrating to the injured neuromasts. (A–C) Live imaging (40×) displays the regions of L3 neuromasts of larvae at the GFP channel, Dsred channel, and bright field (BF) channel and a superimposed image in different groups. Neutrophils (showing both green and yellow fluorescence, indicated by blue arrows) and macrophages (showing only green fluorescence, indicated by white arrows) around the neuromasts could be observed in Tg(coro1a:eGFP; lyz:Dsred2) larvae. They were almost entirely absent from the neuromasts in the control group (A). Many neutrophils and macrophages migrated to injured neuromasts in the CuSO₄ group (B), while fewer neutrophils and macrophages migrated to injured neuromasts in the BRS+CuSO₄ group (C). The image was captured after adding CuSO₄ solution for 1 h. Scale bar represents 50 μm. (D,E) Line charts reveal decreased numbers of neutrophils (D) and macrophages (E) within a radius of 50 μm from the center of neuromasts at different time points after adding CuSO₄ in the BRS+CuSO₄ group (16 ≤ n ≤ 23) compared to the CuSO₄ group (15 ≤ n ≤ 23). The control group (11 ≤ n ≤ 12) was observed at the same time points. The y-axis is a mean of multiple neuromasts (L2, LII3, and L3 neuromasts) from multiple zebrafish. The n values represent the numbers of neuromasts. In (D,E), the asterisk shows the difference between the CuSO₄ group and the BRS+CuSO₄ group. Comparisons were performed using two-way ANOVA, with Tukey’s multiple comparisons test. All error bars show mean ± S.E.M., *** p < 0.001, ** p < 0.01, * p < 0.05.