FIGURE

Figure 2

ID

ZDB-FIG-201003-241

Publication

Cunningham et al., 2020 - Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes

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Figure 2

Suppression of a maternal morphant phenotype by DeMOBS. (A) Two sgRNAs, ctnnb2deMO1 and ctnnb2deMO2 target ctnnb2-MO1 binding site. ctnnb2deMO1 overlaps a Bpu10I restriction enzyme site used for genotyping. A single nucleotide polymorphism resulting in a single base mismatch between the MO and the target sequence is shown in lower case. (B) Confirmation of the (− 4) allele by sequence analysis. (C) Co-injection ctnnb2-MO1 and ctnnb1-MO2 into embryos obtained from four female siblings (two wild type and two heterozygous for the deletion) in blind experiments. (D) ciuffo morphant phenotype resulting from injection of ctnnb1-MO2 and ctnnb2-MO1. (E–H) Residual phenotypes observed in MO-injected embryos from a female heterozygous for the MO-refractive (− 4) mutation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ctnnb2^tpl149/+ ctnnb2^tpl149/+ + MO1-ctnnb2 + MO2-ctnnb1 WT + MO1-ctnnb2 + MO2-ctnnb1
Knockdown Reagents:	MO1-ctnnb2 MO2-ctnnb1
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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