FIGURE

Fig. 7

ID

ZDB-FIG-200924-2

Publication

Zhu et al., 2019 - Zebrafish prmt5 arginine methyltransferase is essential for germ cell development

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Fig. 7

Co-expression of Prmt5 with Vasa or Zili in both ovaries and testis, and disruption of prmt5 leads to reduction of symmetric dimethylarginine levels in Vasa and Zili. (A) Vasa (red), Prmt5 (green) and DNA (DAPI staining; blue) in wild-type zebrafish ovaries (n=6) and testes (n=6) at 1 mpf. Vasa-positive and Prmt5-positive cells are indicated (white arrowheads). (B) Zili (red), Prmt5 (green) and DNA (DAPI staining; blue) in wild-type zebrafish ovaries (n=6) and testis (n=6) at 1 mpf. Zili-positive and Prmt5-possitive cells are indicated (white arrowheads). (C) Vasa (red), Sym11 (symmetric dimethylarginine antibody, green) and DNA (DAPI staining, blue) in prmt5+/+ testes (n=6) and prmt5−/− gonads (n=6) at 1 mpf. Vasa-positive and Sym11-positive cells are indicated (white arrowheads). (D) Zili (red), Sym11 (green) and DNA (DAPI staining, blue) in prmt5+/+ testes (n=6) and prmt5−/− gonad (n=6) at 1 mpf. Zili-positive and SYM11-positive cells are indicated (white arrowheads). Scale bars: 20 μm.

Expression Data

Genes:	ddx4 piwil2 prmt5
Antibodies:	Ab1-sDMA AB2-piwil2 AB2-prmt5
Fish:	AB prmt5^{ihb1994/ihb1994} (AB)
Anatomical Terms:	ovary testis
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prmt5^{ihb1994/ihb1994} (AB)
Observed In:	testis
Stage:	Days 30-44

Phenotype Detail

Acknowledgments

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