FIGURE

Fig 5

ID

ZDB-FIG-200822-5

Publication

Smith et al., 2020 - De novo phosphoinositide synthesis in zebrafish is required for triad formation but not essential for myogenesis

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Fig 5

Localization of PIPs by immunofluorescence in wildtype and <italic>cdipt</italic> mutant zebrafish.

Confocal micrographs showing localization of PIPs is not affected in early larval development of cdipt mutants. (A, A’) visualization of skeletal muscle from live embryos injected with PLCδPH-GFP, a marker for PI(4,5)P₂. There was no obvious difference in expression between wild type (WT) and cdipt mutant embryos. (B-D, B’-D’) Immunostaining with PIP antibodies of myofibers isolated from WT and cdipt mutants. Localization of PI(4,5)P₂(B, B’), PI3P (C, C’) and PI(3,4)P₂(D, D’) is similar in wildtype and cdipt zebrafish. Scale bars = 10μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdipt^{hsc158/hsc158}
Observed In:	skeletal muscle 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate skeletal muscle 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate skeletal muscle 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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