FIGURE

Fig 2

ID

ZDB-FIG-200822-2

Publication

Smith et al., 2020 - De novo phosphoinositide synthesis in zebrafish is required for triad formation but not essential for myogenesis

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Fig 2

Characterization of the <italic>cdipt</italic> mutant phenotype at 6dpf.

A)cdipt mutant zebrafish exhibit a gastrointestinal phenotype with a dark, globular and oversized liver (yellow outline) and a small intestine (yellow arrows), abnormal jaw structure (black arrows), tissue degradation around the cloaca (black arrowhead), and defective ventral fin (yellow arrowhead). B) Representative image of cdipt mutants at 6dpf, showing normal birefringence pattern indistinguishable from WT siblings, indicative of normal sarcomere organization. C) Confocal micrographs showing localization by indirect immunofluorescence of actin (upper panels), DHPR (middle panels) and α-actinin (bottom panels) in the skeletal myofibers. There is no noticeable difference in the localization of these proteins between WT (left column) and cdipt mutant (right column). Insets represent high magnification of areas surrounded by white rectangles. Scale bars = 5 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdipt^{hsc158/hsc158}
Observed In:	cloaca intestine liver mandibular arch skeleton skeletal muscle skeletal muscle sarcomere ventral fin fold
Stage:	Day 6

Phenotype Detail

Acknowledgments

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