FIGURE

Fig 3

ID

ZDB-FIG-200822-3

Publication

Smith et al., 2020 - De novo phosphoinositide synthesis in zebrafish is required for triad formation but not essential for myogenesis

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Fig 3

Skeletal muscle ultrastructure.

A-B) Transmission electron micrographs show normal skeletal muscle ultrastructure in cdipt larvae. T-tubules (insets; black arrow) are apposed by terminal cisternae of sarcoplasmic reticulum (insets; white arrow). C) Diagram illustrating triad structure and features used for measurements: A1 = T-tubule area; A2 and A3 = terminal cisternae (TC) areas; D1 = maximum distance between TCs; * = gap width (distance between TC membrane and T-tubule membrane). D) There is no significant difference in the triad area between WT and cdipt mutant larvae (A1+A2+A3 graph) (n = 36, p = 0.9217). The T-tubule area (A1 graph) is qualitatively slightly smaller in the cdipt mutant than in WT (n = 36; P = 0.5246), whereas the distance between cisternae at maximum distance (D1 graph) (n = 36, p < 0.0001) and the gap width (* graph) (n = 44, p < 0.0001) are significantly smaller in cdipt mutants than in WT. Scale bars = 200 nm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdipt^{hsc158/hsc158}
Observed In:	skeletal muscle cell junctional sarcoplasmic reticulum membrane
Stage:	Day 6

Phenotype Detail

Acknowledgments

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