Figure 1-S3

(a) Comparison between control uninjected and brain-LPS injected in double transgenic four dpf larvae was made at 16 hpi. Fluorescent reporters for liver (fabp10a:BFP) and macrophages (mfap4:tdTomato) were used for in vivo imaging to determine whether ectopic induction of mfap4 may occur in hepatocytes due to LPS activation. Representative single 2 µm z-plane images do not show ectopic expression of mfap4 other than in infiltrating macrophages in the liver (which are stereotypically located near or within liver sinusoids or gaps between hepatocytes) after LPS injection. Coinciding with a lack of macrophage infiltration in the control animals, no mfap4 expression was observed in the liver (demarcated by dotted line). (b) Scatter plot shows analysis of macrophage infiltration in the liver using the double transgenic larvae. Brain-LPS injection causes infiltration of mfap4:tdTomato expressing macrophages but not in the control uninjected animals. (c) Quantification of liver cells expressing mfap4 shows none did. Individual slices through entire z-stack of whole liver were assessed for co-expression of hepatocyte reporter with the mfap4 reporter. In all plots, each symbol represents an independent larva analyzed. Two-tailed student’s t-test was used to determine statistical significance.