FIGURE

FIGURE 4

ID

ZDB-FIG-200619-18

Publication

Varga et al., 2020 - Tissue-Specific Requirement for the GINS Complex During Zebrafish Development

Other Figures

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FIGURE 4

Gins1 deficient and Gins1, Gins2 double-deficient embryos show comparable cell death phenotypes in eyes and tecta. (A,B) The gins1^elu11 frameshift allele created through genome editing shows retinal and tectal apoptosis at 2 dpf, similar to other CMG mutants. (C) Sanger sequencing of gins1^elu11/elu11 embryos shows the presence of the homozygous c.250_251delCT mutation. (D,E) Phenotype of gins1 mutant embryos and their siblings injected with 100 μM gins2MO. (F) Western blot analysis of protein lysates from 2 dpf control and gins1^elu11/elu11 embryos probed with Gins1 and γ-tubulin antibodies. (G) Western blot analysis of protein lysates from wildtype embryos at the indicated stages probed with Gins1 and γ-tubulin antibodies. Scale bar: 250 μm.

Expression Data

Gene:	gins1
Fish:	WT gins1^elu11/elu11
Anatomical Term:	whole organism
Stage Range:	8-cell to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gins1^elu11/elu11 WT + MO1-gins2 gins1^elu11/elu11 + MO1-gins2
Knockdown Reagent:	MO1-gins2
Observed In:	optic tectum optic tectum apoptotic process retina
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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