FIGURE 2

u773 is a loss-of-function allele of gins2. (A) Initial region of Chromosome 18 that segregated with the u773 mutant phenotype. (B) WGS mapping plot of SNP homozygosity on Chromosome 18. (C) Sanger sequencing results aligned to the reference sequence of gins2 for u773 mutants and ekwill controls. (Note that gins2 is on the reverse strand and the sequence is shown in the reverse direction). (D) Multiple sequence alignment of Gins2/Psf2 protein sequences in different species. The red box and asterisk denotes the lysine residue affected in u773 mutants. (E) Protein lysates from uninjected (cntrl) and gins2MO-injected 2 dpf embryos were separated with SDS-PAGE and then subjected to western blotting with Gins2 and γ-tubulin antibodies. (F,G) Phenotype of 2 dpf gins2MO injected embryos and uninjected controls (black arrowhead indicates dead cells). Scale bars: 250 μm. (H) Graph showing the fraction of morphants showing the gins2^u773 phenotype at the indicated gins2MO concentrations.