gins2 functions cell-autonomously and is expressed in proliferating tissues. (A,B) TUNEL staining (red) of wildtype host embryos receiving heterologous cell transplants labeled with membrane-tethered GFP (green) from wildtype and gins2MO-injected embryos. (A’,B’) show magnified views of the boxes in (A,B). (C–I’) Lateral views (C–I) and transverse section (I’) of the wildtype embryos assessed for zygotic gins2 expression with whole mount in situ hybridization at the indicated stages. (J) Western blot analysis of protein lysates from wildtype embryos probed with Gins2 and γ-tubulin antibodies. Scale bars: (A,B) 50 μm; (C–I) 150 μm.
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