FIGURE

Fig. 5

ID

ZDB-FIG-200618-24

Publication

Hehr et al., 2018 - Polarity and morphogenesis of the eye epithelium requires the adhesion junction associated adaptor protein Traf4

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Fig. 5

The traf4a morphant eye phenotype is phenocopied in a traf4a genetic mutant. A. The nucleotide sequence of exon1 of the traf4a gene in wild-type and traf4a-/- fish indicating the 10 bp deletion caused by CRISPR mutagenesis, and wild-type and mutant sequence chromatograms showing the premature stop codon introduced in the mutant by the resulting frameshift. B-G: Dorsal views (B,D-G) of traf4a+/+, traf4a+/- and traf4a-/- 14 ss embryos in a Tg(rx3:GFP) background, and a traf4a-/- embryo in brightfield (C). H-I: Lateral view of 48 hpf eyes. Scale bar in B is 100 µm for panels B, D-I. J-K: Quantitation of mean eye size (J) and % difference between the sizes of the two eyes (K). Number of embryos analyzed is shown. *** p < 0.001, non-parametric Mann-Whitney Rank Sums Test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	traf4a^ca301/ca301
Observed In:	head optic vesicle
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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