ZFIN Image: Hehr et al., 2018, Fig. 5

Image

Figure Caption

Fig. 5 The traf4a morphant eye phenotype is phenocopied in a traf4a genetic mutant. A. The nucleotide sequence of exon1 of the traf4a gene in wild-type and traf4a-/- fish indicating the 10 bp deletion caused by CRISPR mutagenesis, and wild-type and mutant sequence chromatograms showing the premature stop codon introduced in the mutant by the resulting frameshift. B-G: Dorsal views (B,D-G) of traf4a+/+, traf4a+/- and traf4a-/- 14 ss embryos in a Tg(rx3:GFP) background, and a traf4a-/- embryo in brightfield (C). H-I: Lateral view of 48 hpf eyes. Scale bar in B is 100 µm for panels B, D-I. J-K: Quantitation of mean eye size (J) and % difference between the sizes of the two eyes (K). Number of embryos analyzed is shown. *** p < 0.001, non-parametric Mann-Whitney Rank Sums Test.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Adh. Migr.