FIGURE

Figure 1

ID

ZDB-FIG-200501-53

Publication

Liu et al., 2020 - The metalloproteinase Papp-aa controls epithelial cell quiescence-proliferation transition

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Excel spreadsheet containing quantitative data for <xref rid='fig1' ref-type='fig'>Figure 1</xref>.
Papp-aa is highly expressed in NaR cells. (A) Tg(igfbp5a:GFP) fish were raised in E3 embryo medium to 3 days post fertilization (dpf) and transferred to embryo media containing the indicated [Ca²⁺]. Eighteen hours later, NaR cells were isolated by FACS. The levels of papp-aa, papp-ab, and papp-a2 mRNA were measured and shown. Data shown are Mean ± SEM, n = 4. (B–C) NaR cells and other cells in four dpf Tg(igfbp5a:GFP) larvae were separated by FACS. The levels of papp-aa (B) and papp-ab (C) mRNA were measured and shown. *, p<0.05 by unpaired two-tailed t test. n = 3. (D) Whole mount in situ hybridization analysis of papp-aa mRNA in three dpf larvae. HB, hindbrain. pLL, posterior lateral line ganglion. Arrowheads indicate papp-aa mRNA signal in the yolk sac region. A sense cRNA probe was used as a negative control. Scale bar = 0.2 mm. (E) Tg(igfbp5a:GFP) fish of the indicated stages were analyzed by double label staining. Scale bar = 20 µm. Figure 1—source data 1.

Expression Data

Genes:	pappaa pappab
Fish:	mi602Tg
Anatomical Term:	NaK ionocyte
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi602Tg
Observed In:	NaK ionocyte
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife