ZFIN Image: Liu et al., 2020, Figure 1

Image

Figure Caption

Figure 1 Papp-aa is highly expressed in NaR cells.

(A) Tg(igfbp5a:GFP) fish were raised in E3 embryo medium to 3 days post fertilization (dpf) and transferred to embryo media containing the indicated [Ca²⁺]. Eighteen hours later, NaR cells were isolated by FACS. The levels of papp-aa, papp-ab, and papp-a2 mRNA were measured and shown. Data shown are Mean ± SEM, n = 4. (B–C) NaR cells and other cells in four dpf Tg(igfbp5a:GFP) larvae were separated by FACS. The levels of papp-aa (B) and papp-ab (C) mRNA were measured and shown. *, p<0.05 by unpaired two-tailed t test. n = 3. (D) Whole mount in situ hybridization analysis of papp-aa mRNA in three dpf larvae. HB, hindbrain. pLL, posterior lateral line ganglion. Arrowheads indicate papp-aa mRNA signal in the yolk sac region. A sense cRNA probe was used as a negative control. Scale bar = 0.2 mm. (E) Tg(igfbp5a:GFP) fish of the indicated stages were analyzed by double label staining. Scale bar = 20 µm.

Figure 1—source data 1.Excel spreadsheet containing quantitative data for <xref rid='fig1' ref-type='fig'>Figure 1</xref>.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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