Fig 5

(A) Incomplete body axis organization was caused by cspg4 knockdown. The degrees of angle between anterior end of embryo and tailbud (labeled by yellow line) were measured by ImageJ. (B) Quantitative results of the angle between anterior end and tailbud were analyzed (each dot represents one embryo. Control-MO, n = 97; 2 ng MO-e2i2, n = 78; 4 ng MO-e2i2, n = 52; 8 ng MO-e2i2, n = 66). (C) Knockdown cspg4 using translational blocking morpholino also had larger angle between anterior end of embryo and tailbud (control-MO, n = 144; 1 ng MO-start, n = 70; 2 ng MO-start, n = 122; 4 ng MO-start, n = 73). (D) Co-injecting the ineffective dose of MO-e2i2 (1 ng) and MO-start (1 ng) also led to the same phenotype. (E) The angle between anterior end and tailbud was significantly reduced when co-injecting 250 pg cspg4 mRNA with 4 ng cspg4 morpholino (control, n = 63; knockdown, n = 43; overexpress, n = 99; rescue, n = 51). (F) About 12% (18/148) of embryos had fused eyes (indicated by arrowhead) or only one eye (indicated by arrow). In figure (B), (C) and (E), the significant difference between groups were tested by ANOVA followed by Tukey’s multiple comparison. Different letters (a, b or c) indicate significant difference between groups (p ≤ 0.05). Student’s t-test was used in analyzed data in panel (D), ** indicates p ≤ 0.01. Data are presented as mean ±SD.