FIGURE

Fig. 3

ID

ZDB-FIG-200406-148

Publication

Zhao et al., 2020 - Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Cell proliferation, apoptosis, and oxidative & ER stresses in copper-stressed embryos. A Cell proliferation assay by PH3 staining (green dots). B Cell apoptosis assay by TUNEL (red dots) detection. (A, B) sections of embryonic eyes. A10, number of PH3 positive cells per section, B10, average number of apoptotic cells of retinal sections in each group (n > 3, 3–5 sections from each embryo were used for counting the red positive apoptotic cells). C Immunostaining of Caspase3 (green) in retina sections. D Western blot detection with antibody Bcl-2 in copper-stressed embryos. E TEM analysis of retinal cells in copper-stressed embryos at 96 hpf. E1-E6: sagittal slides of retina, red color indicating mitochondria and green color indicating ER. F DCHF-DA assay of embryonic retina. G qRT-PCR detection of ER-stressed genes in embryos at 96 hpf. Scale bars: A1-A9, B1-B9 and C1-C9, 50 μm; E1-E6, 0.5 μm; F1-F3, 100 μm; **, P < 0.01; *, P < 0.05

Expression Data

Genes:	atf4a atf6 ddit3 eif2ak3 ern1 hspa5
Antibody:	Ab5-bcl2
Fish:	AB
Conditions:	chemical treatment: copper(2+) chemical treatment: copper atom
Anatomical Term:	whole organism
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment: copper(2+) chemical treatment: copper atom
Observed In:	retina apoptotic process retina cell population proliferation retina endoplasmic reticulum retina mitochondrial inner membrane retina mitochondrion retina reactive oxygen species whole organism
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Commun. Signal.