FIGURE

Fig. 3

ID

ZDB-FIG-200319-34

Publication

Collins et al., 2019 - Early sarcomere and metabolic defects in a zebrafish pitx2c cardiac arrhythmia model

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Fig. 3

Mitochondrial and metabolic changes in pitx2c−/− adult atria. (A) Quantification of relative mtDNA content in pitx2c+/+ and pitx2c−/− atria by qPCR. (B) Representative image of mitochondrial clusters (highlighted in green) observed in pitx2c−/− adult hearts by TEM (n = 3). (C) qPCR of known PITX2 targets as well as additional metabolic genes in pitx2c+/+ and pitx2c−/− adult atria. Significant down-regulation of cox7c, ppargc1a, foxo1a, pparaa, ucp1, and prdx5 mRNA levels and up-regulation of hcn4 and esrra as well as adipogenic genes fabp11a, adipor2, and zbtb16b mRNA levels are observed in pitx2c−/− atria (C). Error bars correspond to SEM. *P < 0.05 by unpaired t test; **P < 0.01 by unpaired t test.

Expression Data

Genes:	adipor2 cox7c esrra fabp4a foxo1a hcn4 mcu nrf1 pparaa ppargc1a ppargc1b prdx5 sod1 sod2 ucp1 zbtb16b
Fish:	WT pitx2^ups6/ups6
Anatomical Term:	atrium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pitx2^ups6/ups6
Observed In:	atrium atrium mitochondrial chromosome atrium mitochondrion
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA