FIGURE

Fig. 5

ID

ZDB-FIG-200306-120

Publication

Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish

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Fig. 5

Ectopic Bergmann glial cells (BGs) in reln mutants. (A–H) Aberrant localization of BGs in reln mutants. Sagittal section of adult (96 dpf) SAGFF(LF)251A; UAS:GFP brains, which express GFP in BGs, harboring WT (control, n= 5) or homozygous reln mutant (relnΔ7/Δ7, n= 5) alleles were stained with anti-Pvalb7 (magenta) and anti-GFP (green) antibodies. (B-D, F–H) High magnification images of the boxes in A and E. (I) Ratio of the BG (GFP+) area in the GCL to the total BG area in controls and relnΔ7/Δ7mutants. Fluorescence images were captured of every fourth section (14 total sections in each fish). The GFP+ area in the Pvalb7-negative GCL layer was measured and divided by the total GFP+ area in the cerebellum. *p< 0.05 (Mann-Whitney test). Scale bars: 300 μm in A (applies to A and E); 50 μm in B (applies to B-D and F–H).

Expression Data

Genes:	EGFP pvalb7
Antibody:	Ab1-pvalb7
Fish:	nkSAGFFLF251AEt; nkuasgfp1aTg (AB) reln^nub23/nub23; nkSAGFFLF251AEt; nkuasgfp1aTg (AB)
Anatomical Terms:	Bergmann glial cell Purkinje cell cell body Purkinje cell neuron projection
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	reln^nub23/nub23; nkSAGFFLF251AEt; nkuasgfp1aTg (AB)
Observed In:	Bergmann glial cell granular layer corpus cerebelli
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.