FIGURE

Fig. S5

ID

ZDB-FIG-200305-10

Publication

Gjini et al., 2019 - Disruption of asxl1 results in myeloproliferative neoplasms in zebrafish

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Fig. S5

EGFP+ HSPCs in asxl1 +/+, asxl1 +/- and asxl1 -/- embryos at 3 dpf. Knockdown of bim (D-F) and bid (G-I) or overexpression of bcl2 (J-L) each were able to partially rescue HSPC numbers in asxl1 -/- embryos compared with control embryos. This result is expected because mitochondrial intrinsic programmed cell death depends on the combined activities of BH3-only pro-apoptotic proteins and is blocked by prosurvival proteins like bcl2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asxl1^zdf34/zdf34; zf169Tg asxl1^zdf34/zdf34; zf169Tg + MO1-bcl2l11 asxl1^zdf34/zdf34; zf169Tg + MO1-bida
Knockdown Reagents:	MO1-bcl2l11 MO1-bida
Observed In:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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