(A–D) Analysis of proliferative capacity of the adult intestine in celsr1a mutants and age matched wild-type fish (pulse injection and incorporation after 4 hr (red), nuclei counterstained by DAPI. (A, B) Low power view of comparable posterior regions of intestine of wild-type (A) and age and size matched frnt mutants (B). (C, D) Close up of intestinal rugae showing cells incorporating BrdU. (E–H), in situ hybridization of expression of sex-determining region Y-box 2 (sox2) (E, F) and olfactomedin 4 (olfm4). (G, H) genese in adult intestinal epithelia of wild-type (E, G) and celsr1a mutant (F, H) zebrafish. (I) Quantitation of changes in the number of olfm4+ cells observed in mutants; data presented as mean +/- standard deviation, *p<0.05, n = 5 (wt sibling), n = 7 (frnt) (J–K) Proliferative cells (24 hr after BrdU pulse, red) in comparison to celsr1a expression (green, yellow arrowhead) in larval developing intestine at 5dpf in (J) ceslr1aGFP heterozytotes and (K) ceslr1aGFP mutants; DAPI, blue. Insets J’ and J’’ and K’ and K’’are separate channels showing celsr1a expression and Brdu detection, respectively in the same tisse.
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