FIGURE

Fig. 5.

ID

ZDB-FIG-200116-5

Publication

Rissone et al., 2019 - A model for reticular dysgenesis shows impaired sensory organ development and hair cell regeneration linked to cellular stress

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5.

ak2 deficiency impairs IC neuromast formation. (A,B) Chemical treatments of ak2^hg14 mutants and their control siblings (WT) with AG1478 or 6-BIO. (A) Confocal analysis at 5 dpf of trunk regions for untreated, 0.03% DMSO or 3 µM AG1478-treated embryos in the Tg(tnks1bp1:EGFP; atoh1a:dTOM) transgenic background. Scale bars: 50 µm. (B) Alkaline phosphatase staining of 5 dpf ak2^hg14 and control embryos untreated or treated with 0.02% DMSO, 2 µM AG1478 or 0.2 µM 6-BIO compounds. White asterisks indicate IC neuromasts. Scale bars: 50 μm. (C) Quantification of the effect of AG1478 or 6-BIO treatments in inducing the formation of ectopic ICs in ak2^hg14 homozygous mutants and their control siblings. The total number of embryos analyzed per genotype is indicated above each bar.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ak2^hg14/hg14 ak2^hg14/hg14; tnks1bp1^y229Gt; nns8Tg
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: tyrphostin AG 1478
Observed In:	posterior lateral line neuromast
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.