FIGURE

Figure 5

ID
ZDB-FIG-191230-1577
Publication
Johansson et al., 2019 - Dkk1 Controls Cell-Cell Interaction through Regulation of Non-nuclear β-Catenin Pools
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Figure 5

Loss of Dkk1 Increases Cell Adhesion and Polarization

(A and B) Single-plane confocal images of Tg(gsc:GFP) embryos (green axis) at 80% epiboly (EB) stained with phalloidin (red). (A) Prechordal plate leading edge. A stitch-like pattern of actin distribution in cells surrounding the prechordal plate is evident in Dkk1 knock down (KD) embryos (arrowheads). (B) Filamentous actin along the anterior half of the axis. Dashed rectangles indicate the area of higher magnification of the single-plane confocal images shown on the right panels. Filamentous actin is highly concentrated in discrete puncta at the plasma membrane in the paraxial cells of Dkk1 KD embryos (arrowheads) (n = 10–15 for each condition). Scale bar, 20 μm.

(C) Single-plane confocal images of β-catenin antibody-stained Tg(gsc:GFP) embryos at the tail bud (TB) stage show strong patches of endogenous β-catenin (red) along the plasma membrane between connected cells. Dashed squares indicate the area of higher magnification shown on the right side of single-plane confocal images. Control (Ctrl) n = 5 and Dkk1 KD n = 6. Scale bar, 20 μm.
Expression Data
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Bud

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Bud

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 51(6), Johansson, M., Giger, F.A., Fielding, T., Houart, C., Dkk1 Controls Cell-Cell Interaction through Regulation of Non-nuclear β-Catenin Pools, 775-786.e3, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell
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