Figure 7

The locomotor activity of zPnpo morphants at 24 hpf. Embryos injected with 5 ng of zPnpo MO at one- to two-cell stages were co-injected with 800 pg of zpnpo/hPNPO mRNA (columns 4 and 5) or grown in embryo water containing 1 mM of PLP, 1 mM of PN, 0.1 mM of PM, 0.1 mM of PL, or 0.5 mM of GABA (columns 6–10) and recorded for larval spontaneous contraction at 24 hpf. Embryos injected with 10 ng of scrambled control MO were used as control (CTL). Reported are the averages of data from at least three independent repeats with n = 80∼120 embryos for each group and expressed as mean ± SEM. The statistical significance was calculated with one-way ANOVA (Kruskal–Wallis test) by comparing the experimental groups with wild type (*) or with zPnpo MO (#). ^##p < 0.01; ***/^###p < 0.001. MO, morpholino oligonucleotide; PLP, pyridoxal 5′-phosphate; PN, pyridoxine; PM, pyridoxamine; PL, pyridoxal; ANOVA, analysis of variance.