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The impact of knocking down zPnpo. (A) The protein levels of zPnpo in zebrafish embryos injected with 10 ng of scrambled control MO (CTL MO; lane 2), 10 ng of zPnpo MO (MO; lane 3), or zPnpo MO plus 800 pg of zebrafish pnpo mRNA (MO + RNA; lane 4) at 14 hpf and wild-type embryos of the same stage (WT; lane 1) were analyzed with Western blotting (left) and quantified by normalizing with β-actin (right). Shown here are the representative image and average of at least five independent repeats. (B) Dose-dependent decreased survival was observed in zPnpo morphants at 3 dpf. Data presented were the representatives and averages of at least three independent repeats with n = 40∼60 embryos for each group and expressed as mean ± SEM. The statistical significance was calculated with one-tailed Mann–Whitney nonparametric U test by comparing the experimental groups with wild type (*) or with zPnpo MO (#). */#p < 0.05; **p < 0.01; ***p < 0.001. MO, morpholino oligonucleotide.
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