Primary sequence overlap, conservation, and expression of the mir-183/96/182 cluster. (a) Schematic diagram illustrating genomic structure of the mir-183/96/182 cluster. (b) Primary sequence of each miRNA in human, mouse, and zebrafish. The minimal seed sequence is displayed in red text. Nucleotides within the seed region with 100% conservation between component miRNAs and across species are highlighted in yellow. Nucleotides highlighted in black are similarly conserved, but are outside the seed sequence. (c) miRNA expression in zebrafish head tissue during early development was measured by qPCR. For all data points, n = 3. **p < 0.01, ***p < 0.001, ANOVA with Dunnet’s multiple comparisons test, relative to 1dpf.
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