FIGURE

Fig. 2

ID

ZDB-FIG-191118-19

Publication

Lee et al., 2019 - A novel zebrafish model to emulate lung injury by folate deficiency-induced swim bladder defectiveness and protease/antiprotease expression imbalance

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Fig. 2

The tissue integrity and pathology of the swim bladder in wild-type and FD embryos and larvae. Heat-shocked larvae of both wild-type control and Tg( hsp:EGFP-GGH) were harvested at indicated stages post embryogenesis and analyzed for swim bladder development. ( A) Embryos were subjected to WISH with tissues specific probes including Hb9 (epithelial layer), Acta2 (mesenchyme layer, smooth muscle) and Anax5 (outer mesothelium layer). Cross-sections of the embryos after WISH staining revealed comparable intensity and morphology for the tissue layers composed of swim bladders (black arrows) in the early embryos. ( B) Larvae at 5 dpf examined with TUNEL assay for apoptotic cells (arrows) revealed no positive signal in the swim bladder area (circled by dash-line) of both wild-type control and FD larvae. Embryos pre-treated with DNase I served as a positive control. ( C) Larvae at 5 dpf were stained with Sudan black (left) and Oil red O (right) to reveal outlines of swim bladder (black arrows) in larvae. nt, neural tube; sb, swim bladder; WT, wild-type larvae with heat-shock; FD, FD larvae with heat-shock.

Expression Data

Genes:	acta2 anxa5b mnx1
Fish:	cku1Tg
Condition:	heat shock
Anatomical Terms:	tunica externa swim bladder tunica interna swim bladder
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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