ZFIN Image: Lee et al., 2019, Fig. 2

Image

Figure Caption

Fig. 2

The tissue integrity and pathology of the swim bladder in wild-type and FD embryos and larvae. Heat-shocked larvae of both wild-type control and Tg( hsp:EGFP-GGH) were harvested at indicated stages post embryogenesis and analyzed for swim bladder development. ( A) Embryos were subjected to WISH with tissues specific probes including Hb9 (epithelial layer), Acta2 (mesenchyme layer, smooth muscle) and Anax5 (outer mesothelium layer). Cross-sections of the embryos after WISH staining revealed comparable intensity and morphology for the tissue layers composed of swim bladders (black arrows) in the early embryos. ( B) Larvae at 5 dpf examined with TUNEL assay for apoptotic cells (arrows) revealed no positive signal in the swim bladder area (circled by dash-line) of both wild-type control and FD larvae. Embryos pre-treated with DNase I served as a positive control. ( C) Larvae at 5 dpf were stained with Sudan black (left) and Oil red O (right) to reveal outlines of swim bladder (black arrows) in larvae. nt, neural tube; sb, swim bladder; WT, wild-type larvae with heat-shock; FD, FD larvae with heat-shock.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.