FIGURE

Fig. 6

ID

ZDB-FIG-190807-6

Publication

Talbot et al., 2019 - Muscle precursor cell movements in zebrafish are dynamic and require six-family genes

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Fig. 6

Smo function is required during migration for AbFM/AdFM partitioning. (A-D) Confocal projections of six1b:lyn-GFP (green) and mylpfa:mCherry (magenta) transgene expression in embryos fixed at 36 hpf (A,B) or 48 hpf (C,D). When embryos are treated with cyclopamine beginning at 24 hpf, fin buds form and become populated with MMPs; however, few MMPs contribute to the AbFM. At 36 hpf (A,B), we show an individual confocal plane (inset) that highlights defective AbFM/AdFM partitioning. (E,F) Embryos treated with cyclopamine from 24-36 hpf conspicuously lack hgfa expression in the fin bud domain (green arrows). Arrowheads are as described in Fig. 1 legend. Scale bars: 100 µm.

Expression Data

Gene:	hgfa
Fish:	cz3327Tg; oz5Tg
Condition:	chemical treatment by environment: Cyclopamine
Anatomical Term:	pectoral fin bud
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cz3327Tg; oz5Tg
Condition:	chemical treatment by environment: Cyclopamine
Observed In:	middle migratory muscle precursor stream pectoral fin bud primitive pectoral fin abductor
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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