Fig. 6
Smo function is required during migration for AbFM/AdFM partitioning. (A-D) Confocal projections of six1b:lyn-GFP (green) and mylpfa:mCherry (magenta) transgene expression in embryos fixed at 36?hpf (A,B) or 48?hpf (C,D). When embryos are treated with cyclopamine beginning at 24?hpf, fin buds form and become populated with MMPs; however, few MMPs contribute to the AbFM. At 36?hpf (A,B), we show an individual confocal plane (inset) that highlights defective AbFM/AdFM partitioning. (E,F) Embryos treated with cyclopamine from 24-36?hpf conspicuously lack hgfa expression in the fin bud domain (green arrows). Arrowheads are as described in Fig. 1 legend. Scale bars: 100?µm.