Fig. 4

met is required for normal MMP migration. (A-F) Confocal projections of six1b:lyn-GFP (green) and mylpfa:mCherry(magenta) transgene expression in embryos fixed at indicated stages. Compared with DMSO-treated controls, treatment with the Met inhibitor SGX523 causes reduced migratory streams at 36 and 48 hpf and smaller MMP-derived muscles at 72 hpf. (G-J) Confocal projections of phalloidin-labeled wild-type and met mutant embryos at 96 hpf, false-colored to show muscle identity using color code described in Fig. 1. The fin and PHM muscles are shown in lateral view in G and H and the more anteriorly located SHM is shown in ventral view in I and J. Insets in E-H show confocal sections through the fin to distinguish AbFM and AdFM muscles. In met mutants and SGX523-treated embryos, the AbFM is more severely affected than the AdFM and in this example the AbFM is lost entirely. (K) Box plots of 96 hpf wild-type and met mutant MMP-derived muscle measurements. For all plots, WT/Het values are shown in gray and met mutant values are shown in brown. Asterisks indicate significant differences (P<0.01), determined by Tukey–Kramer HSD comparisons after one-way ANOVA; n.s. indicates not significant. See Materials and Methods for statistical details. Measurements were taken on a total of 31 mutants and 29 WT/Het siblings from three separate experiments. Arrowheads are as described in Fig. 1 legend. Scale bars: 100 µm.