Fig. 4

(A) Ventral view of transgenic mylz2:GFP expression in the craniofacial muscles of met^+/+ siblings (n = 5) and met^-/- mutant (n = 5) embryos at 4 dpf. (B) Average GFP⁺sternohyoideus area of met^-/- mutant embryos (n = 6) in proportion to met^+/+ siblings (n = 6) at 4 dpf. (C) Ventral view of transgenic mylz2:EGFP expression in the sternohyoideus of met^+/+ siblings and met^-/- mutant embryos at 4 dpf. (D) Lateral view of transgenic mylz2:GFP expression in the somites of met^+/+ siblings (n = 5) and met^-/-mutant embryos (n = 5) at 4 dpf. (E) Lateral view of met^+/+ siblings (n = 10) and met^-/-mutant larvae (n = 9) fed fluorescent beads at 5 dpf, the average number of beads detected in the stomach is presented in (F). (G) Lateral view of transgenic mylz2:EGFP expression in the oesophagus of met^+/+ (n = 5) siblings and met^-/- (n = 5) mutant embryos at 4 dpf. Error bars indicate S.E.M. Significance was calculated using students t-test where p<0.05 was considered significant, * p<0.05, ** p<0.01, *** p<0.001. Abbreviations: oe: oesophagus; phm: posterior hypaxial muscle; sh: sternohyoideus. Scale bar: 100 μm.