FIGURE

Figure 4

ID

ZDB-FIG-190723-887

Publication

Zhang et al., 2019 - Endoglin is a conserved regulator of vasculogenesis in zebrafish-Implications for hereditary haemorrhagic telangiectasia

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Figure 4

Endoglin knockdown decreased the expression of endothelial markers

(A) RT-PCR for endothelial markers in the Con-MO and eng-MO group, including kdrl, cdh5, hey2, lmo2 and pu.1 (A). Gapdh was used as an internal control. (B) Quantification of mRNA expression in (A) with grey scanning analysis, conducted with ImageJ. (C) WISH for endothelial marker expression in the Con-MO and eng-MO group among 24 hpf embryos (n>30 for each group). The red arrow indicates the region where the endothelial markers were significantly decreased. Scale bars are 200 μm. (D) qPCR for cdh5, dll4, flt4 and kdrl expression in the brain and trunk of the Con-MO and eng-MO group. Gapdh was used as an internal control (error bars, SEM; n=3 biological replicates). A value of P was considered statistically significant (^*P<0.05, ^**P<0.01, ^***P<0.001) for B and D.

Expression Data

Genes:	cdh5 dll4 flt4 hey2 kdrl lmo2 spi1b
Fish:	WT WT + MO1-eng
Knockdown Reagent:	MO1-eng
Anatomical Terms:	brain dorsal aorta endothelial cell posterior cardinal vein trunk whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-eng
Knockdown Reagent:	MO1-eng
Observed In:	brain dorsal aorta endothelial cell posterior cardinal vein trunk whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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