FIGURE

Figure 3

ID

ZDB-FIG-190723-468

Publication

Tobia et al., 2019 - Atypical Chemokine Receptor 3 Generates Guidance Cues for CXCL12-Mediated Endothelial Cell Migration

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Figure 3

Effect of ackr3b knockdown on arterial/venous differentiation and lateral dorsal aorta (LDA) bifurcation. (A–F) WISH analysis of the arterial marker ephrin-B2a(A,B) and venous markers ephb4a(C,D) and flt4(E,F) expression was performed at 28 hpf on zebrafish embryos injected with std-MO (A,C,E) or ackr3b-MO1 (B,D,F). Arterial and venous marker expression is normal in ackr3b morphants when compared to controls. (G,H) WISH analysis of cadherin 5 expression was performed at 30 hpf on zebrafish embryos injected with std-MO (G) or ackr3b-MO1 (H) to investigate LDA formation. Note the interruption of LDA in ackr3b morphants [asterisk in H]. The number of embryos presenting the showed phenotype in respect to the total number of analyzed embryos is shown in each panel. Scale bar: 100 μm.

Expression Data

Genes:	cdh5 efnb2a ephb4a flt4
Fish:	AB AB + MO9-ackr3b
Knockdown Reagent:	MO9-ackr3b
Anatomical Terms:	artery blood vasculature dorsal aorta posterior cardinal vein vein
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO9-ackr3b
Knockdown Reagent:	MO9-ackr3b
Observed In:	lateral dorsal aorta
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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