Figure 7

Theoretical Predictions Support the Role of Basal Protrusions in Patterning Differentiation Through Delta-Notch-Mediated Lateral Inhibition

(A–C) Histograms of the distributions of the distance between successive differentiation events predicted by theoretical model assuming a random distribution of differentiation events (A) (mean ± SD, 40.90 ± 21.55 μm), assuming lateral inhibition signaling occurs through basal protrusions of wild-type length (B) (mean ± SD, 54.53 ± 18.92 μm), or, assuming lateral inhibition signaling occurs through basal protrusions of lamc1 length (C) (mean ± SD, 46.32 ± 18.68 μm).

(D) Box-and-whisker plots of the distance between successive differentiation events under various in vivo conditions and model predictions. The Kolmogorov-Smirnov test was used to compare wild-type and lamc1 distribution (p-value = 0.000167), wild-type and predicted random distribution (p-value < E⁻¹²), lamc1 mutant and predicted random distribution (p-value = 9.6E⁻⁷), wild-type and predicted distribution when basal protrusions of wild-type length convey lateral inhibition (p-value = 0.121), and lamc1 mutant and predicted distributions when shorter and slower (lamc1 length and dynamics) basal protrusions convey lateral inhibition (p-value = 0.181).

(E) Predicted relationship between the average maximum length of basal protrusions and the mean distance between sequential differentiation events.