PUBLICATION
Basal Protrusions Mediate Spatiotemporal Patterns of Spinal Neuron Differentiation
- Authors
- Hadjivasiliou, Z., Moore, R.E., McIntosh, R., Galea, G.L., Clarke, J.D.W., Alexandre, P.
- ID
- ZDB-PUB-190619-10
- Date
- 2019
- Source
- Developmental Cell 49: 907-919.e10 (Journal)
- Registered Authors
- Alexandre, Paula, Clarke, Jon
- Keywords
- basal protrusions, lateral inhibition, live imaging, neuronal differentiation, spatiotemporal pattern, spinal cord, zebrafish
- MeSH Terms
-
- Animals
- Body Patterning*
- Cell Communication
- Cell Differentiation*
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Intracellular Signaling Peptides and Proteins/genetics
- Intracellular Signaling Peptides and Proteins/metabolism
- Laminin/genetics
- Laminin/metabolism*
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Motor Neurons/cytology*
- Motor Neurons/metabolism
- Neurogenesis
- Signal Transduction
- Spatio-Temporal Analysis
- Spinal Cord/cytology*
- Spinal Cord/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 31211994 Full text @ Dev. Cell
Citation
Hadjivasiliou, Z., Moore, R.E., McIntosh, R., Galea, G.L., Clarke, J.D.W., Alexandre, P. (2019) Basal Protrusions Mediate Spatiotemporal Patterns of Spinal Neuron Differentiation. Developmental Cell. 49:907-919.e10.
Abstract
During early spinal cord development, neurons of particular subtypes differentiate with a sparse periodic pattern while later neurons differentiate in the intervening space to eventually produce continuous columns of similar neurons. The mechanisms that regulate this spatiotemporal pattern are unknown. In vivo imaging in zebrafish reveals that differentiating spinal neurons transiently extend two long protrusions along the basal surface of the spinal cord before axon initiation. These protrusions express Delta protein, consistent with the hypothesis they influence Notch signaling at a distance of several cell diameters. Experimental reduction of Laminin expression leads to smaller protrusions and shorter distances between differentiating neurons. The experimental data and a theoretical model support the proposal that neuronal differentiation pattern is regulated by transient basal protrusions that deliver temporally controlled lateral inhibition mediated at a distance. This work uncovers a stereotyped protrusive activity of newborn neurons that organize long-distance spatiotemporal patterning of differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping