FIGURE

Fig. 7.

ID

ZDB-FIG-190723-2592

Publication

Hu et al., 2019 - Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy

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Fig. 7.

Autophagy inducer RAPA and block of Hh signaling co-inhibited osteoblast mineralization. (A) Larvae at 3 dpf were exposed to 10 µM cyA, 10 µM RAPA or cyA plus RAPA, with larvae collected for Alizarin Red staining at 6 dpf. Blue circle, op; white circle, cb. (B–D) Embryos were injected with 50 µM SHHMO, Gli2MO, Gli2Mis, PTCH1MO and PTCH1Mis at the 1–4 cell stage, DMSO control group and injected group larvae were treated with 10 µM RAPA, with larvae collected at 6 dpf for Alizarin Red staining. Yellow circle, bop; green circle, hm; blue rectangle, rib. Scale bars: 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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