ZFIN Image: Hu et al., 2019, Fig. 7.

Image

Figure Caption

Fig. 7.

Autophagy inducer RAPA and block of Hh signaling co-inhibited osteoblast mineralization. (A) Larvae at 3 dpf were exposed to 10 µM cyA, 10 µM RAPA or cyA plus RAPA, with larvae collected for Alizarin Red staining at 6 dpf. Blue circle, op; white circle, cb. (B–D) Embryos were injected with 50 µM SHHMO, Gli2MO, Gli2Mis, PTCH1MO and PTCH1Mis at the 1–4 cell stage, DMSO control group and injected group larvae were treated with 10 µM RAPA, with larvae collected at 6 dpf for Alizarin Red staining. Yellow circle, bop; green circle, hm; blue rectangle, rib. Scale bars: 50 µm.

Acknowledgments

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