Fig 4

(A) The density of atp1a1a.1⁺-Na⁺-K⁺-ATPase-rich (NaR) cells was reduced in yolk extensions of embryos injected with both klf4 MO1 and MO2 (c) as compared to uninjected wild types (a) and control embryos injected with klf4 5mmMO2 (b). The density of atp1a1a.1⁺-NaR cells was increased in yolk extensions of embryos injected with klf4 (g) mRNA, as compared to embryos injected with the same amount of LacZ (f) mRNA, or uninjected wild type (e) embryos. Quantification of results from (a-c) and (e-g) are shown in (d) and (h), respectively. (B) The density of atp6v1aa⁺- H⁺-ATPase-rich (HR) cells was reduced in yolk extensions of embryos injected with both klf4 MO1 and MO2 (c) as compared to uninjected wild types (a) and control embryos injected with klf4 5mmMO2 (b). The density of atp6v1aa⁺-HR cells was increased in yolk extensions of embryos injected with klf4 (g) mRNA, as compared to embryos injected with the same amount of LacZ (f) mRNA, or uninjected wild-type (e) embryos. Quantification of results from (a-c) and (e-g) are shown in (d) and (h), respectively. Statistical significance was determined by Student’s t-test. ***p < 0.001. Scale bar, 300 μm. Error bars indicate standard error.